MYBPC3 Polyclonal antibody

MYBPC3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19977-1-AP

Synonyms

Myosin-binding protein C, cardiac-type, MYBPC 3, C-protein, cardiac muscle isoform, Cardiac MyBP-C, Cardiac MyBP C

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Validation Data Gallery

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  WB analysis using 19977-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 19977-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 19977-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 19977-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 19977-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 19977-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 19977-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue, human heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

19977-1-AP targets MYBPC3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin binding protein C, cardiac
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 141-150 kDa
• GenBank Accession Number: NM_000256
• Gene Symbol: MYBPC3
• Gene ID (NCBI): 4607
• RRID: AB_10858232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4). The antibody detects the full length form and the degradation bands around 28-54 kDa in SDS-PAGE.(PMID: 22076249)

Publications

Species	Application	Title
mouse	WB	FASEB J; Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.; Authors - Julia Straubinger
human	IHC	Front Cardiovasc Med; Case Report: Identification of the First Synonymous Variant of Myosin Binding Protein C3 (c.24A>C, p.P8P) Altering RNA Splicing in a Cardiomyopathy and Sudden Cardiac Death Case.; Authors - Jie-Yuan Jin
mouse	WB	Front Cardiovasc Med; Hypercontractile cardiac phenotype in mice overexpressing the regulatory subunit PR72 of protein phosphatase 2A; Authors - Julius R Herting