MYBBP1A Monoclonal antibody, PBS Only

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  WB analysis using 67996-1-Ig (same clone as 67996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67996-1-Ig (same clone as 67996-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67996-1-Ig (same clone as 67996-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67996-1-Ig (MYBBP1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

MYBBP1A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67996-1-PBS

Clone No.1H2D10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FLJ37886, MYB binding protein (P160) 1a, Myb binding protein 1A, MYBBP1A, P160, PAP2

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Product Information

67996-1-PBS targets MYBBP1A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYBBP1A fusion protein Ag6008
• Full Name: MYB binding protein (P160) 1a
• Calculated Molecular Weight: 149 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC050546
• Gene Symbol: MYBBP1A
• Gene ID (NCBI): 10514
• RRID: AB_2918745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9BQG0
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].