MX2 Polyclonal antibody

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  WB analysis using 13278-1-AP

  Untreated and IFN beta treated THP-1 cell lysates were subjected to SDS PAGE followed by western blot with 13278-1-AP (MX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13278-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13278-1-AP (MX2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MX2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13278-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

p78-related protein, p78 related protein, Myxovirus resistance protein 2, MXB, Interferon-regulated resistance GTP-binding protein MxB

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Tested Applications

• Positive WB detected in: THP-1 cells, IFN beta treated THP-1 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

13278-1-AP targets MX2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MX2 fusion protein Ag4020
• Full Name: myxovirus (influenza virus) resistance 2 (mouse)
• Calculated Molecular Weight: 715 aa, 82 kDa
• Observed Molecular Weight: 70-82 kDa
• GenBank Accession Number: BC035293
• Gene Symbol: MX2
• Gene ID (NCBI): 4600
• RRID: AB_3669157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20592
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myxovirus resistance 2 (MX2, also known as MxB) is a member of both the dynamin family and the family of large GTPases. MX2 is an interferon-stimulated gene that inhibits the nuclear import of HIV-1 and interacts with the viral capsid and cellular nuclear transport machinery. MX2 mediates the effects of IFN signaling in a cell type and context-dependent manner that can influence cells sensitivity to MAPK pathway targeted therapies. MX2 is also associated with the tumorigenesis process in melanoma(PMID: 33734579).

Publications

Species	Application	Title
human	WB	Cell; Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit.; Authors - Nathan Meade
human	WB	J Virol; mTOR Dysregulation by Vaccinia Virus F17 Controls Multiple Processes with Varying Roles in Infection.; Authors - Nathan Meade
human	WB	J Virol; YTHDF2 Is Downregulated in Response to Host Shutoff Induced by DNA Virus Infection and Regulates Interferon-Stimulated Gene Expression; Authors - Charles R Hesser
human	WB	Nat Commun; The poxvirus F17 protein counteracts mitochondrially orchestrated antiviral responses; Authors - Nathan Meade