MX1 Recombinant antibody, PBS Only

MX1 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2L19

Cat no : 82677-1-PBS

Synonyms

IFI 78K, IFI78, Interferon induced protein p78, MX, MX1, MxA, Myxovirus resistance protein 1

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Validation Data Gallery

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  WB analysis using 82677-1-RR (same clone as 82677-1-PBS)

  Untreated and IFN alpha treated A549 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 82677-1-RR (MX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82677-1-PBS targets MX1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MX1 fusion protein Ag4692
• Full Name: Myxovirus resistance protein 1
• Calculated Molecular Weight: 662 aa, 76 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC032602
• Gene Symbol: MX1
• Gene ID (NCBI): 4599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN.