MVP/LRP Polyclonal antibody

• Featured Product
• KD/KO Validated

MVP/LRP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 16478-1-AP

Synonyms

MVP, LRP, VAULT1, Major vault protein, Lung resistance-related protein

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Validation Data Gallery

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  WB analysis using 16478-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16478-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 16478-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 16478-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16478-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16478-1-AP (MVP/LRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16478-1-AP

  IP result of anti-MVP/LRP (IP:16478-1-AP, 4ug; Detection:16478-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human lung cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16478-1-AP (MVP/LRP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16478-1-AP (MVP/LRP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16478-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16478-1-AP (MVP/LRP antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 16478-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MVP/LRP antibody (16478-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, RAW264.7, human brain tissue, mouse lung tissue, rat lung tissue, SH-SY5Y cells
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human breast cancer tissue, human testis tissue, human skin tissue, human lung cancer tissue, human kidney tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

16478-1-AP targets MVP/LRP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MVP/LRP fusion protein Ag9593
• Full Name: major vault protein
• Calculated Molecular Weight: 893 aa, 99 kDa
• Observed Molecular Weight: 99-104 kDa
• GenBank Accession Number: BC015623
• Gene Symbol: LRP
• Gene ID (NCBI): 9961
• RRID: AB_2147597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].

Publications

Species	Application	Title
mouse	WB	Cell; The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.; Authors - Deniz Simsek
human	WB,IHC,IF,IP	Drug Resist Updat; CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis; Authors - Jing Wu ; KD Validated
human	WB	Nat Commun; MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression.; Authors - Yun Teng ; KO Validated
mouse	WB	Front Cell Dev Biol; Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes.; Authors - Xinlei Li
human	WB	Front Cell Dev Biol; Major Vault Protein (MVP) Associated With BRAF V600E Mutation Is an Immune Microenvironment-Related Biomarker Promoting the Progression of Papillary Thyroid Cancer via MAPK/ERK and PI3K/AKT Pathways.; Authors - Xubin Dong ; KD Validated
mouse	WB	Cell Commun Signal; Differential RNA packaging into small extracellular vesicles by neurons and astrocytes.; Authors - Xuan Luo

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