MUS81 Polyclonal antibody

• Featured Product
• KD/KO Validated

MUS81 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11018-1-AP

Synonyms

EC:3.1.22.-

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Validation Data Gallery

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  WB analysis using 11018-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11018-1-AP (MUS81 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11018-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11018-1-AP (MUS81 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 11018-1-AP

  Immunofluorescent analysis of MCF-7 cells, using MUS81 antibody 11018-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HepG2 cells, NIH/3T3 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11018-1-AP targets MUS81 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUS81 fusion protein Ag1476
• Full Name: MUS81 endonuclease homolog (S. cerevisiae)
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 52-72 kDa
• GenBank Accession Number: BC009999
• Gene Symbol: MUS81
• Gene ID (NCBI): 80198
• RRID: AB_2147130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).

Publications

Species	Application	Title
human	WB,IHC	Cell; EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.; Authors - Antonio Marzio ; KD Validated
	WB	Elife; 53BP1 and BRCA1 control pathway choice for stalled replication restart.; Authors - Yixi Xu