MUPCDH Polyclonal antibody

MUPCDH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25619-1-AP

Synonyms

CDHR5, MU PCDH, Mu protocadherin, MUCDHL, mucin like protocadherin, MUPCDH

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Validation Data Gallery

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  WB analysis of HEK-293 using 25619-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25619-1-AP (MUPCDH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25619-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human kidney using 25619-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human small intestine using 25619-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 25619-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25619-1-AP is MUPCDH Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUPCDH fusion protein Ag22537
• Full Name: mucin-like protocadherin
• Calculated Molecular Weight: 845 aa, 88 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC038405
• Gene Symbol: MUPCDH
• Gene ID (NCBI): 53841
• RRID: AB_2880164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUPCDH (Mu-protocadherin), also known as MUCDHL (Mucin and cadherin-like protein) and CDHR5 (Cadherin-related family member 5), is a member of the cadherin superfamily. It is involved in calcium-dependent cell adhesion and may have additional functions in terminally differentiated epithelia. The MUPCDH gene maps to 11p15.5, with highest expression in kidney, liver, colon and small intestine. Alternatively spliced transcript variants encoding different isoforms have been described. (PMID: 12167596; 21839066)

Publications

Species	Application	Title
mouse	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu