MUM1 Polyclonal antibody

MUM1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 12682-1-AP

Synonyms

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere, PWWP3A

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Validation Data Gallery

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  WB analysis using 12682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12682-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12682-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12682-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12682-1-AP

  IP result of anti-MUM1 (IP:12682-1-AP, 4ug; Detection:12682-1-AP 1:300) with Jurkat cells lysate 4000ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HT-1080 cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12682-1-AP targets MUM1 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUM1 fusion protein Ag3371
• Full Name: melanoma associated antigen (mutated) 1
• Calculated Molecular Weight: 18 kDa, 79 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC008098
• Gene Symbol: MUM1
• Gene ID (NCBI): 84939
• RRID: AB_10642694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.

Publications

Species	Application	Title
mouse	WB	Nature; Post-translational control of beige fat biogenesis by PRDM16 stabilization; Authors - Qiang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aurelie (Verified Customer) (09-01-2019)

Lysats (20µg) from Mouse cortex were loaded in a 8% SDS-PAGE and immunoblotted using anti-MUM1 antibody ( 1/1000) over-night at 4°C.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Cortex

MUM1 Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex (Cat no:12682-1-AP)