MUL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MUL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 16133-1-AP

Synonyms

C1orf166, GIDE, MAPL, MUL1, MULAN, RING finger protein 218, RNF218, RP11 401M16.2

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Validation Data Gallery

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  WB analysis of HeLa using 16133-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16133-1-AP (MUL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16133-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16133-1-AP (MUL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16133-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16133-1-AP (MUL1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16133-1-AP targets MUL1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUL1 fusion protein Ag9119
• Full Name: mitochondrial E3 ubiquitin ligase 1
• Calculated Molecular Weight: 352 aa, 40 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC010101
• Gene Symbol: MUL1
• Gene ID (NCBI): 79594
• RRID: AB_2147111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial ubiquitin ligase 1 (MUL1),MAPL, MULAN, or gGIDE, was identified as an E3 protein ligase by three independent groups. Work in mammalian systems shows that MUL1 has small ubiquitin-like modifier (SUMO) ligase activity, stabilizing Drp1, or ubiquitin ligase activity, degrading Mfn. MUL1 expression in mammalian cells results in smaller and more fragmented mitochondria.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu
rat	WB	Oxid Med Cell Longev; Inhibition of Prostaglandin E2 Receptor EP3 Attenuates Oxidative Stress and Neuronal Apoptosis Partially by Modulating p38MAPK/FOXO3/Mul1/Mfn2 Pathway after Subarachnoid Hemorrhage in Rats; Authors - Yu Liu
mouse	WB	Life Sci; Trichostatin A inhibits skeletal muscle atrophy induced by cigarette smoke exposure in mice.; Authors - Jingjing Ding
human,mouse	WB	Eur J Pharmacol; Mitochondrial E3 ubiquitin ligase 1 promotes brain injury by disturbing mitochondrial dynamics in a rat model of ischemic stroke.; Authors - Kai-Di Ren ; KD Validated
rat	CoIP	Can J Physiol Pharmacol; Upregulation of mitochondrial E3 ubiquitin ligase 1 in rat heart contributes to ischemia/reperfusion injury.; Authors - Shi-Jing Wang
rat	WB,IP	J Ginseng Res; Ginsenoside compound K protects against cerebral ischemia/reperfusion injury via Mul1/Mfn2-mediated mitochondrial dynamics and bioenergy; Authors - Qingxia Huang