MUC5B Polyclonal antibody

MUC5B Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28118-1-AP

Synonyms

MG1, MUC5, MUC5B, MUC9



Tested Applications

Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28118-1-AP targets MUC5B in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC5B fusion protein Ag27886
Full Name mucin 5B, oligomeric mucus/gel-forming
Calculated Molecular Weight 596 kDa
GenBank Accession NumberNM_002458
Gene Symbol MUC5B
Gene ID (NCBI) 727897
RRIDAB_2881067
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MUC5B antibody 28118-1-APDownload protocol
IF protocol for MUC5B antibody 28118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Transl Med

Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.

Authors - Qian Hao
ratWB

J Ethnopharmacol

Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats

Authors - San Fu
pigIF

Front Immunol

Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium

Authors - Kohtaro Fukuyama
humanWB

PLoS One

Assessment of polymeric mucin-drug interactions

Authors - Hisanao Kishimoto