MUC5AC Polyclonal antibody

MUC5AC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30408-1-AP

Synonyms

MUC5

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Validation Data Gallery

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  WB analysis using 30408-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 30408-1-AP (MUC5AC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 30408-1-AP

  Immunohistochemical analysis of paraffin-embedded normal stomach slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 30408-1-AP

  Immunohistochemical analysis of paraffin-embedded normal stomach slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 30408-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 30408-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30408-1-AP targets MUC5AC in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUC5AC fusion protein Ag30190
• Full Name: mucin 5AC, oligomeric mucus/gel-forming
• Calculated Molecular Weight: 586 kDa
• Observed Molecular Weight: 290 kDa
• GenBank Accession Number: NM_001304359
• Gene Symbol: MUC5AC
• Gene ID (NCBI): 4586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC5AC (Mucin-5AC ) is a gel-forming glycoprotein of the gastric and respiratory epithelium. It can protect mucous membranes from infection and chemical damage by binding to inhaled microorganisms and particles, which are then cleared by the mucociliary system. (PubMed:14535999,PubMed:14718370)