MUC5AC Polyclonal antibody

MUC5AC Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20725-1-AP

Synonyms

MUC5

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Validation Data Gallery

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  IHC staining of human stomach using 20725-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20725-1-AP (MUC5AC antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 20725-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20725-1-AP (MUC5AC antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat stomach using 20725-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 20725-1-AP (MUC5AC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 20725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 20725-1-AP (MUC5AC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 20725-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20725-1-AP (MUC5AC antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human stomach tissue, mouse stomach tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20725-1-AP targets MUC5AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mucin 5AC, oligomeric mucus/gel-forming
• Calculated Molecular Weight: 527 kDa
• GenBank Accession Number: NM_017511
• Gene Symbol: MUC5AC
• Gene ID (NCBI): 4586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC5AC is a member of the mucin family, a heterogeneous group of 21 abundant, high molecular weight O-glycoproteins that can be either secreted or membrane bound. Multiple cysteine-rich domains in both N- and C-terminal regions of MUC5AC are responsible for its disulfide-mediated polymerization, which is critical for gel-forming properties. (PMID: 26751774, PMID: 27845339)

Publications

Species	Application	Title
human,mouse	WB	Mol Immunol; Knockdown of Brg1 reduced mucus secretion in HDM stimulated airway inflammation; Authors - Maozhu Xu