MUC1/CA15-3 C-terminal Recombinant antibody

MUC1/CA15-3 C-terminal Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240190G10

Cat no : 83311-4-RR

Synonyms

CA15-3, CA153, MUC1, CA15 3, CA 15-3

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Validation Data Gallery

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  WB analysis using 83311-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83311-4-RR (MUC1/CA15-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 83311-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83311-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83311-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

• 

  Affinity and Kinetic Characterization of 83311-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83311-4-RR against Human CA15-3/MUC1 were performed. The affinity constant is 63.7 pM.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse lung tissue
• Positive IHC detected in: human stomach cancer tissue, human ovary cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83311-4-RR targets MUC1/CA15-3 C-terminal in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MUC1/CA15-3 C-terminal fusion protein Ag20366
• Full Name: mucin 1, cell surface associated
• Calculated Molecular Weight: 1264 aa, 123 kDa
• Observed Molecular Weight: 23-30 kDa
• GenBank Accession Number: BC120975
• Gene Symbol: MUC1/CA15-3
• Gene ID (NCBI): 4582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.