MTX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11610-1-AP

Synonyms

Metaxin-2, metaxin 2

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Validation Data Gallery

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  WB analysis of mouse heart using 11610-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse kidney using 11610-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11610-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse heart using 11610-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse heart using 11610-1-AP

  IP result of anti-MTX2 (IP:11610-1-AP, 3ug; Detection:11610-1-AP 1:300) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 11610-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11610-1-AP (MTX2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11610-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11610-1-AP (MTX2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11610-1-AP

  Immunofluorescent analysis of HepG2 cells, using MTX2 antibody 11610-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse kidney tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

11610-1-AP targets MTX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTX2 fusion protein Ag2181
• Full Name: metaxin 2
• Calculated Molecular Weight: 263 aa, 30 kDa
• Observed Molecular Weight: 25-29 kDa
• GenBank Accession Number: BC017271
• Gene Symbol: MTX2
• Gene ID (NCBI): 10651
• RRID: AB_10859665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian ; KD Validated
human	WB	Cell Death Dis; EB virus-induced ATR activation accelerates nasopharyngeal carcinoma growth via M2-type macrophages polarization.; Authors - Bo Zhang
mouse	WB	Hepatology; Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury; Authors - Li Chen
human	WB	Cell Death Differ; Sam50-Mic19-Mic60 axis determines mitochondrial cristae architecture by mediating mitochondrial outer and inner membrane contact; Authors - Junhui Tang
mouse	IF	J Ovarian Res; Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes; Authors - Chuanxin Zhang
mouse	WB	Cell Rep; Mitochondrial cristae architecture protects against mtDNA release and inflammation; Authors - Baiyu He ; KO Validated