MTX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTX1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15529-1-AP

Synonyms

metaxin 1, MTX, MTX1, MTXN

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Validation Data Gallery

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  WB analysis using 15529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15529-1-AP (MTX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15529-1-AP (MTX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 15529-1-AP

  IP result of anti-MTX1 (IP:15529-1-AP, 4ug; Detection:15529-1-AP 1:700) with MCF-7 cells lysate 1280ug.

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, Jurkat cells, HeLa cells
• Positive IP detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Product Information

15529-1-AP targets MTX1 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTX1 fusion protein Ag7685
• Full Name: metaxin 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC001906
• Gene Symbol: MTX1
• Gene ID (NCBI): 4580
• RRID: AB_2148416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METAXINs are proteins located in the outer membrane of the mammalian mitochondrion. METAXIN 1 was characterized firstly and was found to play a role in the import of mitochondrial precursor proteins. It may also play a role in the assembly of b-barrel proteins into the outer membrane METAXIN1 was reported to be essential for survival of the postimplantation mouse embryo. METAXIN 1 has multiple transcripts. Several isoforms of METAXIN 1 exist due to the alternative splicing, with the predicted MW of 51, 48 and 36 kDa, respectively. Metaxin 1 interacts with metaxin 2, a novel related protein associated with the mammalian mitochondrial outer membrane and form complex in the mitochondrion outer membrane.

Publications

Species	Application	Title
human	WB,IP	Int J Biol Sci; Genome-scale CRISPRa screening identifies MTX1 as a contributor for sorafenib resistance in hepatocellular carcinoma by augmenting autophagy.; Authors - Li Li ; KD Validated
mouse	WB	Hepatology; Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury; Authors - Li Chen
human	WB	J Cell Sci; Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission; Authors - Stephen M Coscia ; KD Validated