MTMR9 Recombinant antibody

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  WB analysis using 83694-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83694-4-RR (MTMR9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83694-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR9 antibody (83694-4-RR, Clone: 240167G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 83694-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 83694-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83694-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83694-4-RR against Human MTMR9 were performed. The affinity constant is below 1 pM.

MTMR9 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83694-4-RR

Clone No.240167G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

myotubularin related protein 9, MTMR8, LIP STYX, Inactive phosphatidylinositol 3-phosphatase 9, C8orf9

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, HeLa cells, HepG2 cells, U2OS cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83694-4-RR targets MTMR9 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MTMR9 fusion protein Ag34134
• Full Name: myotubularin related protein 9
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: ~60 kDa
• GenBank Accession Number: BC022003
• Gene Symbol: MTMR9
• Gene ID (NCBI): 66036
• RRID: AB_3671297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96QG7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTMR9 (Myotubularin-related protein 9) acts as an adapter for myotubularin-related phosphatases (PMID: 19038970; 22647598). MTMR9 can increase lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate and MTMR6 binding affinity for phosphorylated phosphatidylinositols (PMID: 19038970; 22647598). It can also increase MTMR8 catalytic activity towards phosphatidylinositol 3-phosphate and stabilize MTMR8 protein levels (PMID: 22647598).