MTMR12 Polyclonal antibody

• 

  WB analysis using 17552-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17552-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17552-1-AP

  WB result of MTMR12 antibody (17552-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and MTMR12 knockout HeLa cells.

• 

  WB analysis of K-562 using 17552-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17552-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17552-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17552-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17552-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 17552-1-AP

  IP result of anti-MTMR12 (IP:17552-1-AP, 4ug; Detection:17552-1-AP 1:500) with K-562 cells lysate 2400 ug.

• Featured Product
• KD/KO Validated

MTMR12 Polyclonal Antibody for WB, IP, ELISA

Cat No. 17552-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

KIAA1682, Inactive phosphatidylinositol 3-phosphatase 12, 3-phosphatase adapter protein, 3-PAP, 3 phosphatase adapter protein

---

Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, mouse brain tissue, mouse lung tissue, HeLa cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

17552-1-AP targets MTMR12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTMR12 fusion protein Ag11710
• Full Name: myotubularin related protein 12
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC057393
• Gene Symbol: MTMR12
• Gene ID (NCBI): 54545
• RRID: AB_2147824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C0I1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IP	FASEB Bioadv; In search of alternatively spliced alpha-Klotho Kl1 protein in mouse brain; Authors - Liping Li