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MTIF3 Polyclonal antibody

MTIF3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14219-1-AP

Synonyms

IF 3(Mt), IF 3mt, IF3(mt), IF3mt, MTIF3



Tested Applications

Positive WB detected inA431 cells, human preadipocyte cells, HT-29 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, mouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 7 publications below

Product Information

14219-1-AP targets MTIF3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTIF3 fusion protein Ag5457
Full Name mitochondrial translational initiation factor 3
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC046166
Gene Symbol MTIF3
Gene ID (NCBI) 219402
RRIDAB_10638621
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTIF3, also named as DC38, belongs to the IF-3 family. MTIF3 encodes a 29 kDa protein that promotes formation of the initiation complex on the mitochondrial 55S ribosome, thereby playing an active role in initiation of translation. Like bacterial IF3, MTIF3 is believed to bind first to the small mitoribosomal subunit to keep it dissociated from the large subunit during initiation. After binding of MTIF3, mRNA and formylated initiator methionyl-tRNA (fMet-tRNAifMet) bind to the small mitoribosomal subunit. The large subunit then joins the small subunit to form an elongation-competent ribosome (PMID:20887776, 31350787).

Protocols

Product Specific Protocols
WB protocol for MTIF3 antibody 14219-1-APDownload protocol
IHC protocol for MTIF3 antibody 14219-1-APDownload protocol
IP protocol for MTIF3 antibody 14219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler
mouseWB

EMBO J

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Authors - Nicola Ferreira
humanWB

Sci Rep

Initiation Factor 3 is Dispensable For Mitochondrial Translation in Cultured Human Cells.

Authors - Ivan V Chicherin

Clin Immunol

Novel minor HLA DR associated antigens in type 1 diabetes.

Authors - Denise Müller
ratWB

Sci Total Environ

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects

Authors - Dongjie Li
humanWB

Elife

Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function

Authors - Mi Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Mi (Verified Customer) (02-10-2023)

It works pretty well! We tried different antibodies from other suppliers, and this one gives a specific band, as we expected from the knockout samples. Although, we did observe some non-specific bands, having a knockout control would validate the target band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: adipocytes