MTHFR Recombinant antibody, PBS Only

MTHFR Uni-rAb^TM Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

240348C12

Cat no : 83372-7-PBS

Synonyms

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 240348C12

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Validation Data Gallery

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  WB analysis using 83372-7-RR (same clone as 83372-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83372-7-RR (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83372-7-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83372-7-RR (same clone as 83372-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTHFR Recombinant antibody (83372-7-RR, Clone:240348C12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83372-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83372-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83372-7-RR against Human MTHFR were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83372-7-PBS is MTHFR Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MTHFR fusion protein Ag14970
• Full Name: 5,10-methylenetetrahydrofolate reductase (NADPH)
• Calculated Molecular Weight: 656 aa, 75 kDa
• GenBank Accession Number: BC053509
• Gene Symbol: MTHFR
• Gene ID (NCBI): 4524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.