MTHFR Recombinant antibody, PBS Only (Capture)

MTHFR Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240348C3

Cat no : 83372-5-PBS

Synonyms

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 240348C3

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00372-1

  Sandwich ELISA standard curve of MP00372-1, Human MTHFR Recombinant Matched Antibody Pair - PBS only. 83372-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14970. 83372-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP00372-1

  K-562, Hela and HepG2 cell lysates were measured. The human MTHFR concentration of detected samples was determined to be 88.86 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate, 57.89 ng/mL (based on a 3.2 mg/mL extract load) in Hela cell lysate and 55.55 ng/mL (based on a 5 mg/mL extract load) in HepG2 cell lysate.

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  Cytometric bead array standard curve of MP00372-1

  Cytometric bead array standard curve of MP00372-1, MTHFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83372-5-PBS. Detection antibody: 83372-4-PBS. Standard: Ag14970. Range: 0.78-100 ng/mL.

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Product Information

83372-5-PBS targets MTHFR as part of a matched antibody pair:

MP00372-1: 83372-5-PBS capture and 83372-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MTHFR fusion protein Ag14970
• Full Name: 5,10-methylenetetrahydrofolate reductase (NADPH)
• Calculated Molecular Weight: 656 aa, 75 kDa
• GenBank Accession Number: BC053509
• Gene Symbol: MTHFR
• Gene ID (NCBI): 4524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.