MTHFR Monoclonal antibody, PBS Only

MTHFR Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7A3

Cat no : 66612-1-PBS

Synonyms

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 1F7A3

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Validation Data Gallery

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  WB analysis of LNCaP using 66612-1-Ig (same clone as 66612-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66612-1-Ig (same clone as 66612-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66612-1-Ig (same clone as 66612-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66612-1-Ig (same clone as 66612-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66612-1-Ig (same clone as 66612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66612-1-Ig (MTHFR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66612-1-Ig (same clone as 66612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66612-1-Ig (MTHFR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66612-1-Ig (same clone as 66612-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTHFR antibody (66612-1-Ig, Clone: 1F7A3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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Product Information

66612-1-PBS targets MTHFR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTHFR fusion protein Ag14970
• Full Name: 5,10-methylenetetrahydrofolate reductase (NADPH)
• Calculated Molecular Weight: 656 aa, 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC053509
• Gene Symbol: MTHFR
• Gene ID (NCBI): 4524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

5,10-Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in the folate metabolic pathway with a key role in generating methyl groups. MTHFR reduces 5,10-methylenetetrahydrofolate (5,10-methyleneTHF) to 5-methylTHF, the primary carbon donor for methionine production from homocysteine. In humans and mice studies, mutations and polymorphisms in MTHFR are associated with impaired spermatogenesis and male infertility. It has been reported that MTHFR is highly phosphorylated under unperturbed conditions and T34 is the priming phosphorylation site. MTHFR gene has two promoters and isoform (70 kDa and 77 kDa). Although the major form of MTHFR is 77kDa in most tissues, a second isoform of 70kDa was observed after western blotting of human fetal liver and porcine liver. (PMID: 24769206, PMID: 34128976, PMID: 18615588)