MTHFD1L Polyclonal antibody

• Featured Product
• KD/KO Validated

MTHFD1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (1)

Cat no : 16113-1-AP

Synonyms

MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3

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Validation Data Gallery

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  WB analysis using 16113-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16113-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16113-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16113-1-AP

  IP result of anti-MTHFD1L (IP:16113-1-AP, 4ug; Detection:16113-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human liver cancer using 16113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16113-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16113-1-AP (MTHFD1L antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, COLO 320 cells, mouse ovary tissue, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 18 publications below
• IHC: See 2 publications below

Product Information

16113-1-AP targets MTHFD1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTHFD1L fusion protein Ag9036
• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
• Calculated Molecular Weight: 978 aa, 106 kDa
• Observed Molecular Weight: 106 kDa
• GenBank Accession Number: BC017477
• Gene Symbol: MTHFD1L
• Gene ID (NCBI): 25902
• RRID: AB_2250974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.; Authors - Saara Forsström
human	WB	Cell Metab; Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability.; Authors - Won Dong Lee
human	WB	Nat Metab; Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells; Authors - Alanna C Green
human, mouse	WB	Cell Metab; Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.; Authors - Joni Nikkanen
human	WB, IHC	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui ; KD Validated
human	WB	Nat Commun; Increased formate overflow is a hallmark of oxidative cancer.; Authors - Johannes Meiser

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Furkan Emre (Verified Customer) (02-21-2022)

I am satisfied with the antibody. It worked as I was expecting from it. I would buy it again. I also add a figure showing chemiluminescence detection of MTHFD1L using your antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human ES2 cells

MTHFD1L Antibody Western Blot validation (1:1000 dilution) in Human ES2 cells (Cat no:16113-1-AP)