MTHFD1 Monoclonal antibody, PBS Only

MTHFD1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B1G3

Cat no : 67670-1-PBS

Synonyms

MTHFC, MTHFD, MTHFD1

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Validation Data Gallery

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  WB analysis using 67670-1-Ig (same clone as 67670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67670-1-Ig (MTHFD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67670-1-PBS targets MTHFD1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTHFD1 fusion protein Ag24553
• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC009806
• Gene Symbol: MTHFD1
• Gene ID (NCBI): 4522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)