MTBP Polyclonal antibody

MTBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28356-1-AP

Synonyms

hMTBP, Mdm2 binding protein, MDM2BP, MTBP

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Validation Data Gallery

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  WB analysis of U2OS using 28356-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28356-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 28356-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28356-1-AP (MTBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28356-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28356-1-AP (MTBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U2OS cells, K-562 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28356-1-AP targets MTBP in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTBP fusion protein Ag28393
• Full Name: Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 38 kDa, 110 kDa
• GenBank Accession Number: BC013136
• Gene Symbol: MTBP
• Gene ID (NCBI): 27085
• RRID: AB_2881118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Gene; Circular RNA Circ_0000119 promotes gastric cancer progression via circ_0000119/miR-502-5p/MTBP axis; Authors - Mengyan Xie