MTAP Polyclonal antibody

• Featured Product
• KD/KO Validated

MTAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (2)

Cat no : 11475-1-AP

Synonyms

MTAPase, MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 11475-1-AP

  WB result of MTAP antibody (11475-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTAP transfected HEK-293 cells.

• 

  WB analysis using 11475-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11475-1-AP

  Various lysates from MTAP-expressing cells (Raji, NIH3T3, HeLa, and HEK293) and MTAP deficient cells (MCF7 and A549) were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11475-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11475-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11475-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11475-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11475-1-AP (MTAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11475-1-AP (MTAP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11475-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTAP antibody (11475-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Raji cells, mouse heart tissue, human heart tissue, NIH/3T3 cells, HeLa cells, HT-29 cells, C6 cells
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 20 publications below

Product Information

11475-1-AP targets MTAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTAP fusion protein Ag2051
• Full Name: methylthioadenosine phosphorylase
• Calculated Molecular Weight: 283 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC018625
• Gene Symbol: MTAP
• Gene ID (NCBI): 4507
• RRID: AB_2147094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

Publications

Species	Application	Title
human	WB	J Clin Invest; SHARPIN-mediated regulation of protein arginine methyltransferase 5 controls melanoma growth.; Authors - Hironari Tamiya
human	WB,IHC	Nat Commun; MTAP deficiency creates an exploitable target for antifolate therapy in 9p21-loss cancers.; Authors - Omar Alhalabi
human	IHC	Nat Commun; Pharmacological polyamine catabolism upregulation with methionine salvage pathway inhibition as an effective prostate cancer therapy.; Authors - Hayley C Affronti
human	IHC	Clin Cancer Res; Homozygous deletion of MTAP gene as a poor prognosticator in gastrointestinal stromal tumors.; Authors - Huang Hsuan-Ying HY
human	WB	Oncoimmunology; Suppressive effects of tumor cell-derived 5'-deoxy-5'-methylthioadenosine on human T cells.; Authors - Frederik C Henrich
human	IHC	J Pathol; Methylthioadenosine phosphorylase and activated insulin-like growth factor-1 receptor/insulin receptor: potential therapeutic targets in chordoma.; Authors - Sommer Josh J

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Satyendra (Verified Customer) (07-16-2021)

I have marked the knockout as KO

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293

MTAP Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:11475-1-AP)

FH

Hua (Verified Customer) (10-21-2020)

Observed band at the right size with a clean background; strong signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse liver lysate

MTAP Antibody Western Blot validation (1:2000 dilution) in mouse liver lysate (Cat no:11475-1-AP)