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  • KD/KO Validated

MTA3 Polyclonal antibody

MTA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14682-1-AP

Synonyms

mta1, MTA 3, Metastasis-associated protein MTA3



Tested Applications

Positive WB detected inHepG2 cells, mouse thymus tissue, Jurkat cells, MCF-7 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14682-1-AP targets MTA3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTA3 fusion protein Ag6400
Full Name metastasis associated 1 family, member 3
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 62 kDa, 68 kDa
GenBank Accession NumberBC053631
Gene Symbol MTA3
Gene ID (NCBI) 57504
RRIDAB_2298003
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

Protocols

Product Specific Protocols
WB protocol for MTA3 antibody 14682-1-APDownload protocol
IHC protocol for MTA3 antibody 14682-1-APDownload protocol
IF protocol for MTA3 antibody 14682-1-APDownload protocol
IP protocol for MTA3 antibody 14682-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Stem Cell

The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development.

Authors - Jinzhong Qin
mouse

Mol Cell

The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression.

Authors - Susanne Bornelöv
humanIHC

Oncotarget

Metastasis-associated protein 3 in colorectal cancer determines tumor recurrence and prognosis.

Authors - Yi Huang
humanWB,IHC

PLoS One

Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer.

Authors - Li Haiying H
  • KD Validated
humanIHC

J Gastroenterol Hepatol

Overexpression of the metastasis-associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma.

Authors - Chuanxi Wang
humanWB,IHC

Tumour Biol

MTA3 regulates malignant progression of colorectal cancer through Wnt signaling pathway.

Authors - Taiwei Jiao