MTA2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MTA2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G12G4

Cat no : 66195-1-PBS

Synonyms

MTA1L1, MTA1 L1 protein, Metastasis-associated protein MTA2, Metastasis-associated 1-like 1, Metastasis associated 1 like 1

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Validation Data Gallery

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  WB analysis using 66195-1-Ig (same clone as 66195-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66195-1-Ig (MTA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66195-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66195-1-Ig (same clone as 66195-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66195-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66195-1-Ig (same clone as 66195-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66195-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66195-1-Ig (same clone as 66195-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66195-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66195-1-Ig (same clone as 66195-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66195-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66195-1-Ig (same clone as 66195-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MTA2 antibody (66195-1-Ig, Clone: 2G12G4 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66195-1-PBS in a different storage buffer formulation.

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Product Information

66195-1-PBS targets MTA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTA2 fusion protein Ag11713
• Full Name: metastasis associated 1 family, member 2
• Calculated Molecular Weight: 668 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC053650
• Gene Symbol: MTA2
• Gene ID (NCBI): 9219
• RRID: AB_2877118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.