MTA2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTA2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17554-1-AP

Synonyms

MTA1-L1 protein, MTA1L1, MTA1 L1 protein, Metastasis-associated protein MTA2, Metastasis-associated 1-like 1

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Validation Data Gallery

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  WB analysis using 17554-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17554-1-AP

  WB result of MTA2 antibody (17554-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA2 transfected HEK-293 cells.

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  WB analysis of mouse thymus using 17554-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17554-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17554-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17554-1-AP (MTA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 17554-1-AP

  IP result of anti-MTA2 (IP:17554-1-AP, 4ug; Detection:17554-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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  IHC staining of rat colon using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 17554-1-AP (MTA2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 17554-1-AP (MTA2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 17554-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17554-1-AP (MTA2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse thymus tissue, HEK-293 cells
• Positive IP detected in: mouse thymus tissue
• Positive IHC detected in: rat lung tissue, human heart tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

17554-1-AP targets MTA2 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTA2 fusion protein Ag11713
• Full Name: metastasis associated 1 family, member 2
• Calculated Molecular Weight: 668 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC053650
• Gene Symbol: MTA2
• Gene ID (NCBI): 9219
• RRID: AB_10638601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.

Publications

Species	Application	Title
human	WB	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	EMBO Rep; miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell level.; Authors - Dapeng Wang
human	WB	Cell Mol Gastroenterol Hepatol; Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.; Authors - Gui-Qi Zhu ; KD Validated
	CoIP,ChIP	Cancer Sci; MTA2 promotes the metastasis of esophageal squamous cell carcinoma via EIF4E-Twist feedback loop.; Authors - Su-Li Dai
human	WB	Nucleic Acids Res; CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development; Authors - Xue Chen