mouse lung tissue were subjected to SDS PAGE followed by western blot with 11053-1-AP (RON, MST1R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 11053-1-AP (RON, MST1R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 11053-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11053-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
RON (also referred to as MST1R, or CD136) is a receptor tyrosine kinase that belongs to the MET proto-oncogene family. The precursor RON is produced as a glycosylated, single chain that is cleaved into a 185-kDa heterodimer of α (35 kDa) and β (150 kDa) subunits connected by a disulfide bond (PMID: 8062829). It is activated upon binding to its ligand, macrophage-stimulating protein (MSP, also known as MST1 and HGFL). RON signalling is essential for embryonic development and regulates many physiological processes including cell survival, migration and differentiation.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 11053-1-AP (RON, MST1R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lung cancer using 11053-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11053-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RON, MST1R Polyclonal antibody
Catalog Number
11053-1-AP
Citations
2
Dilutions
WB : 1:500-1:1000 IHC : 1:50-1:500
Applications
WB, IHC, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
