MSRB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MSRB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 17629-1-AP

Synonyms

CBS 1, CBS1, CGI 131, MSRB, MSRB2, PILB

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Validation Data Gallery

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  WB analysis using 17629-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17629-1-AP (MSRB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 17629-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17629-1-AP (MSRB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17629-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17629-1-AP (MSRB2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 17629-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17629-1-AP (MSRB2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, mouse heart tissue, rat brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below

Product Information

17629-1-AP targets MSRB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSRB2 fusion protein Ag11881
• Full Name: methionine sulfoxide reductase B2
• Calculated Molecular Weight: 182 aa, 20 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC018030
• Gene Symbol: MSRB2
• Gene ID (NCBI): 22921
• RRID: AB_2148380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methionine sulfoxide reductase B2(MSRB2), is a 182-amino acid protein with an N-terminal mitochondrial targeting signal, a catalytic cysteine, and 2 zinc-coordinating CxxC motifs. MSRB can catalyze the stereospecific reduction of methionine-R-sulfoxides to methionines. MSRB2 protects mitochondrial integrity and cell survival by scavenging reactive oxygen species.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Loss of methionine sulfoxide reductases increases resistance to oxidative stress.; Authors - Lo Lai
human	WB	Front Pharmacol; 3-Mercaptopyruvate Sulfurtransferase, Not Cystathionine β-Synthase Nor Cystathionine γ-Lyase, Mediates Hypoxia-Induced Migration of Vascular Endothelial Cells.; Authors - Beibei Tao ; KD Validated
rat	WB	J Med Food; Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age.; Authors - Hui Li
rat	WB	Food Sci Biotechnol; Methionine strengthens anti-inflammation of rice protein via depressing NF-κB activation and stimulating Msr expression in rats fed cholesterol-enriched diets.; Authors - Zhengxuan Wang
rat	WB	J Food Biochem; Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.; Authors - Zhengxuan Wang
rat	WB	Mol Omics; Rbm20 ablation is associated with changes in the expression of titin-interacting and metabolic proteins.; Authors - Eli J Larson