MSRA Polyclonal antibody, PBS Only

MSRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14547-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human kidney using 14547-1-AP (same clone as 14547-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14547-1-AP (MSRA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

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  WB analysis using 14547-1-AP (same clone as 14547-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14547-1-AP (MSRA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 14547-1-AP (same clone as 14547-1-PBS)

  IP result of anti-MSRA (IP:14547-1-AP, 4ug; Detection:14547-1-AP 1:600) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 14547-1-AP (same clone as 14547-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14547-1-AP (MSRA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

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Product Information

14547-1-PBS targets MSRA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSRA fusion protein Ag6053
• Full Name: methionine sulfoxide reductase A
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 24-26 kDa
• GenBank Accession Number: BC054033
• Gene Symbol: MSRA
• Gene ID (NCBI): 4482
• RRID: AB_2148366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Methionine sulfoxide reductase A(MSRA), is a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine.MSRA has some isoforms with MW of 19~26 kDa. MSRA functions in the repair of oxidatively damaged proteins to restore biological activity. Transfection of MSRA reduced colony formation and the invasiveness of HCC cell lines.