MSMB Polyclonal antibody

MSMB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15888-1-AP

Synonyms

Beta microseminoprotein, HPC13, IGBF, Immunoglobulin binding factor, microseminoprotein, beta, MSMB, MSP, MSPB, PN44, PRPS, PRSP, PSP, PSP 94, PSP94, Seminal plasma beta inhibin

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Validation Data Gallery

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  WB analysis using 15888-1-AP

  Human urine samples were subjected to SDS PAGE followed by western blot with 15888-1-AP (MSMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 15888-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15888-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15888-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15888-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 15888-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 15888-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15888-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15888-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human urine sample
• Positive IHC detected in: human lung tissue, human prostate hyperplasia tissue, human kidney tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15888-1-AP targets MSMB in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSMB fusion protein Ag8674
• Full Name: microseminoprotein, beta-
• Calculated Molecular Weight: 114 aa, 13 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC005257
• Gene Symbol: MSMB
• Gene ID (NCBI): 4477
• RRID: AB_2878197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.