MS4A12 Polyclonal antibody

MS4A12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13293-1-AP

Synonyms

Membrane-spanning 4-domains subfamily A member 12

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Validation Data Gallery

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  WB analysis using 13293-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13293-1-AP (MS4A12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13293-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13293-1-AP (MS4A12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13293-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13293-1-AP (MS4A12 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 13293-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13293-1-AP (MS4A12 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13293-1-AP targets MS4A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MS4A12 fusion protein Ag3968
• Full Name: membrane-spanning 4-domains, subfamily A, member 12
• Calculated Molecular Weight: 267 aa, 28 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC029793
• Gene Symbol: MS4A12
• Gene ID (NCBI): 54860
• RRID: AB_10596915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.