MRRF Polyclonal antibody

MRRF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12357-2-AP

Synonyms

RRF, Ribosome-releasing factor, mitochondrial, Ribosome-recycling factor, mitochondrial, mtRRF, MRFF

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Validation Data Gallery

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  WB analysis using 12357-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12357-2-AP (MRRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12357-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12357-2-AP (MRRF antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A431 using 12357-2-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MRRF antibody (12357-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

12357-2-AP targets MRRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRRF fusion protein Ag3021
• Full Name: mitochondrial ribosome recycling factor
• Calculated Molecular Weight: 262 aa, 29 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC013049
• Gene Symbol: MRRF
• Gene ID (NCBI): 92399
• RRID: AB_2144259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.; Authors - Takumi Yokokawa
mouse	WB	Physiol Rep; Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.; Authors - Takumi Yokokawa
		EMBO J; Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism; Authors - Ying Shu
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li
rat	WB	J Hazard Mater; ZLN005 alleviates PBDE-47 induced impairment of mitochondrial translation and neurotoxicity through PGC-1α/ERRα axis; Authors - Zhiyuan Tian