MRPS30 Polyclonal antibody

MRPS30 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18441-1-AP

Synonyms

MRP S30, MRPS30, PAP, PDCD9, S30mt

---

Validation Data Gallery

• 

  WB analysis of human myotube protein using 18441-1-AP

  WB result of MRPS30 antibody (18441-1-AP, 1:1000) with with 15µg of control human myotube protein by Max Pohjanpelto, University of Helsinki.

• 

  WB analysis of HeLa using 18441-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18441-1-AP (MRPS30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver mitochondria using 18441-1-AP

  mouse liver mitochondria tissue were subjected to SDS PAGE followed by western blot with 18441-1-AP (MRPS30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 18441-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18441-1-AP (MRPS30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: human myotube protein, HeLa cells, mouse liver mitochondria tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 18441-1-AP is MRPS30 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS30 fusion protein Ag13271
• Full Name: mitochondrial ribosomal protein S30
• Calculated Molecular Weight: 439 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC007735
• Gene Symbol: MRPS30
• Gene ID (NCBI): 10884
• RRID: AB_2878544
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation; Authors - Liang Wang
human	WB	Nat Commun; P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance; Authors - Xiangli Jiang