MRPS18B Polyclonal antibody

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  WB analysis using 16139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 16139-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16139-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16139-1-AP

  IP result of anti-MRPS18B (IP:16139-1-AP, 4ug; Detection:16139-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of A549 using 16139-1-AP

  1x10^6 A549 cells were intracellularly stained with 0.4 ug MRPS18B Polyclonal antibody (16139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MRPS18B Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 16139-1-AP

Publications(57)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Synonyms

MRPS, MRP S18 b, MRP S18 2, HSPC183, C6orf14

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Tested Applications

• Positive WB detected in: A549 cells, mouse liver tissue, rat heart tissue, HEK-293 cells, MCF-7 cells, PC-3 cells, SH-SY5Y cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 52 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

16139-1-AP targets MRPS18B in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS18B fusion protein Ag9198
• Full Name: mitochondrial ribosomal protein S18B
• Calculated Molecular Weight: 258 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC005373
• Gene Symbol: MRPS18B
• Gene ID (NCBI): 28973
• RRID: AB_2146368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y676
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.

Publications

Species	Application	Title
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
	WB	Mol Cell; A kinetic dichotomy between mitochondrial and nuclear gene expression processes; Authors - Erik McShane
	WB,CoIP	Cell Host Microbe; Inducible Germline IgMs Bridge Trypanosome Lytic Factor Assembly and Parasite Recognition.; Authors - Joseph Verdi
human	WB	Cell Metab; The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.; Authors - Antonicka Hana H
human	WB	Nat Struct Mol Biol; Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18.; Authors - Xingqian Zhang
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia