Various lysates were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 16139-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat heart tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16139-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16139-1-AP
IP result of anti-MRPS18B (IP:16139-1-AP, 4ug; Detection:16139-1-AP 1:1000) with HeLa cells lysate 2800ug.
IP result of anti-MRPS18B (IP:16139-1-AP, 4ug; Detection:16139-1-AP 1:1000) with HeLa cells lysate 2800ug.
IHC staining of human breast cancer using 16139-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16139-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 16139-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human breast cancer using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 40x lens).
FC experiment of A549 using 16139-1-AP
1x10^6 A549 cells were intracellularly stained with 0.4 ug MRPS18B Polyclonal antibody (16139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 A549 cells were intracellularly stained with 0.4 ug MRPS18B Polyclonal antibody (16139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected in
A549 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:12000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.
Various lysates were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 16139-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16139-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 16139-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16139-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 16139-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 16139-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 16139-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 16139-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of HeLa using 16139-1-AP
IP result of anti-MRPS18B (IP:16139-1-AP, 4ug; Detection:16139-1-AP 1:1000) with HeLa cells lysate 2800ug.
FC (INTRA) Figures
FC experiment of A549 using 16139-1-AP
1x10^6 A549 cells were intracellularly stained with 0.4 ug MRPS18B Polyclonal antibody (16139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
MRPS18B Polyclonal antibody
Catalog Number
16139-1-AP
Citations
57
Dilutions
WB : 1:2000-1:12000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet