MRPS16 Recombinant antibody

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  WB analysis of HeLa using 82741-2-RR

  WB result of MRPS16 antibody (82741-2-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS16 transfected HeLa cells.

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  WB analysis using 82741-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82741-2-RR (MRPS16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82741-2-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82741-2-RR (MRPS16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82741-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82741-2-RR against Human MRPS16 were performed. The affinity constant is 0.616 nM.

• Featured Product
• KD/KO Validated

MRPS16 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 82741-2-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MRP S16, COXPD2, CGI-132, CGI132, CGI 132

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, HT-1080 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82741-2-RR targets MRPS16 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MRPS16 fusion protein Ag9749
• Full Name: mitochondrial ribosomal protein S16
• Calculated Molecular Weight: 137 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC021106
• Gene Symbol: MRPS16
• Gene ID (NCBI): 51021
• RRID: AB_3086523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y3D3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.