MRPP3 Monoclonal antibody

MRPP3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G9A10

Cat no : 68168-1-Ig

Synonyms

KIAA0391, MRPP3

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Validation Data Gallery

• 

  WB analysis using 68168-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (MRPP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68168-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (KIAA0391 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68168-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (KIAA0391 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells, pig liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68168-1-Ig targets MRPP3 in WB, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

• Tested Reactivity: Human, Mouse, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MRPP3 fusion protein Ag15629
• Full Name: KIAA0391
• Calculated Molecular Weight: 583 aa, 67 kDa
• Observed Molecular Weight: 65-67 kDa
• GenBank Accession Number: BC032221
• Gene Symbol: MRPP3
• Gene ID (NCBI): 9692
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694)