MRPL23 Polyclonal antibody

MRPL23 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11706-1-AP

Synonyms

MRP-L23, MRP L23, Large ribosomal subunit protein uL23m, L23mt, L23MRP



Tested Applications

Positive WB detected inA2780 cells, HEK-293 cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 7 publications below

Product Information

11706-1-AP targets MRPL23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL23 fusion protein Ag2220
Full Name mitochondrial ribosomal protein L23
Calculated Molecular Weight 153 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC027710
Gene Symbol MRPL23
Gene ID (NCBI) 6150
RRIDAB_10694422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPL23 antibody 11706-1-APDownload protocol
IHC protocol for MRPL23 antibody 11706-1-APDownload protocol
IF protocol for MRPL23 antibody 11706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Authors - Tara R Richman
humanWB

Nucleic Acids Res

METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.

Authors - Lindsey Van Haute
mouseWB

Aging Cell

Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.

Authors - Tara R Richman
mouseWB

J Cell Biol

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Authors - Uwe Richter
mouseWB

PLoS Genet

Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction.

Authors - Tara R Richman
humanWB

Genomics Proteomics Bioinformatics

Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.

Authors - Ruona Shi