MRPL14 Polyclonal antibody

MRPL14 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15040-1-AP

Synonyms

MRP-L14, MRP L32, MRP L14, Large ribosomal subunit protein uL14m, L32mt

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Validation Data Gallery

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  WB analysis of HEK-293 using 15040-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15040-1-AP (MRPL14 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IF Staining of HEK-293 using 15040-1-AP

  Immunofluorescent analysis of HEK-293 cells using 15040-1-AP (MRPL14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15040-1-AP targets MRPL14 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL14 fusion protein Ag6808
• Full Name: mitochondrial ribosomal protein L14
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC065005
• Gene Symbol: MRPL14
• Gene ID (NCBI): 64928
• RRID: AB_2878102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
HUMAN	WB	EMBO Mol Med; SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.; Authors - Alexandre Janer