MRP1 Polyclonal antibody

MRP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27825-1-AP

Synonyms

ABC29, ABCC, ABCC1, GS X, Leukotriene C(4) transporter, LTC4 transporter, MRP, MRP1

---

Validation Data Gallery

• 

  WB analysis using 27825-1-AP

  37°C incubated HeLa cells were subjected to SDS PAGE followed by western blot with 27825-1-AP (MRP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 27825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27825-1-AP (ABCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27825-1-AP (ABCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: 37°C incubated HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27825-1-AP targets MRP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRP1 fusion protein Ag27048
• Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 1
• Calculated Molecular Weight: 172 kDa
• Observed Molecular Weight: 180-190 kDa
• GenBank Accession Number: NM_004996
• Gene Symbol: MRP1
• Gene ID (NCBI): 4363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Multidrug resistant-associated protein 1 (MRP1, also known as ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; EYA4 reduces chemosensitivity of osteosarcoma to doxorubicin through DNA damage repair; Authors - Wei Heng