MRAS Polyclonal antibody

MRAS Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14213-1-AP

Synonyms

Ras related protein R Ras3, Ras related protein M Ras, R RAS3, muscle RAS oncogene homolog, M RAs

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Validation Data Gallery

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  WB analysis using 14213-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14213-1-AP (MRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14213-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14213-1-AP (MRAS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14213-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14213-1-AP (MRAS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14213-1-AP

  IP result of anti-MRAS (IP:14213-1-AP, 4ug; Detection:14213-1-AP 1:800) with mouse brain tissue lysate 2400 ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse lung tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

14213-1-AP targets MRAS in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRAS fusion protein Ag5433
• Full Name: muscle RAS oncogene homolog
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC047690
• Gene Symbol: MRAS
• Gene ID (NCBI): 22808
• RRID: AB_10950895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Cell Rep; Mutation-induced LZTR1 polymerization provokes cardiac pathology in recessive Noonan syndrome; Authors - Alexandra Viktoria Busley
human	WB	Stem Cell Res; Generation of a genetically-modified induced pluripotent stem cell line harboring a Noonan syndrome-associated gene variant MRAS p.G23V; Authors - Alexandra Viktoria Busley