MPRIP Polyclonal antibody

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  WB analysis of SH-SY5Y using 20040-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 20040-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 20040-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of SKOV-3 using 20040-1-AP

  IP result of anti-MPRIP (IP:20040-1-AP, 4ug; Detection:20040-1-AP 1:500) with SKOV-3 cells lysate 2000ug.

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  IHC staining of human ovary using 20040-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 20040-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse ovary using 20040-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 20040-1-AP (MPRIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20040-1-AP

  Immunofluorescent analysis of Hela cells, using MPRIP antibody 20040-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

MPRIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20040-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Rho-interacting protein 3, p116Rip, Myosin phosphatase Rho-interacting protein, M-RIP, MRIP

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, A2780 cells, SKOV-3 cells
• Positive IP detected in: SKOV-3 cells
• Positive IHC detected in: mouse ovary tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

20040-1-AP targets MPRIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin phosphatase Rho interacting protein
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 116-140 kDa
• GenBank Accession Number: NM_015134
• Gene Symbol: MPRIP
• Gene ID (NCBI): 23164
• RRID: AB_10694433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6WCQ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP.

Publications

Species	Application	Title
human	WB,IF	Glia; Ermin is a p116RIP -interacting protein promoting oligodendroglial differentiation and myelin maintenance.; Authors - Shan Wang
human	WB	RNA; DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells; Authors - Maya David
human	WB	J Cancer; High Expression of lncRNA AFAP1-AS1 Promotes the Progression of Colon Cancer and Predicts Poor Prognosis.; Authors - Hao Bo