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MPP8 Recombinant antibody, PBS Only

MPP8 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242205B5

Cat no : 84719-4-PBS

Synonyms

MPHOSPH8, Two hybrid-associated protein 3 with RanBPM, Twa3, M-phase phosphoprotein 8, HSMPP8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MPP8 Recombinant antibody (84719-4-RR)

    WB analysis using 84719-4-RR (same clone as 84719-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84719-4-RR (MPP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84719-4-PBS in a different storage buffer formulation.

Product Information

84719-4-PBS targets MPP8 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MPP8 fusion protein Ag10268
Full Name M-phase phosphoprotein 8
Calculated Molecular Weight 860 aa, 97 kDa
Observed Molecular Weight 97-105 kDa
GenBank Accession NumberBC003542
Gene Symbol MPP8
Gene ID (NCBI) 54737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a)