MPP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

MPP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 17710-1-AP

Synonyms

Protein PALS1, Protein associated with Lin-7 1, PALS1, Membrane protein, palmitoylated 5, MAGUK p55 subfamily member 5

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Validation Data Gallery

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  WB analysis using 17710-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17710-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17710-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17710-1-AP

  IP result of anti-MPP5 (IP:17710-1-AP, 3ug; Detection:17710-1-AP 1:1000) with mouse brain tissue lysate 8000ug.

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  IHC staining of mouse brain using 17710-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17710-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 17710-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MPP5 antibody (17710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: DU 145 cells, human brain tissue, Y79 cells, HeLa cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 7 publications below
• IF: See 16 publications below
• IP: See 1 publications below

Product Information

17710-1-AP targets MPP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MPP5 fusion protein Ag12010
• Full Name: membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
• Calculated Molecular Weight: 675 aa, 77 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC095485
• Gene Symbol: MPP5
• Gene ID (NCBI): 64398
• RRID: AB_2282012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.

Publications

Species	Application	Title
mouse	IF	Neuron; Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.; Authors - Divya Jayaraman
mouse	IF	Neuron; Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells.; Authors - Robert Beattie
human	WB,IP	Nat Commun; CD13 orients the apical-basal polarity axis necessary for lumen formation.; Authors - Li-Ting Wang
mouse	IF	Dev Cell; Multicellular rosettes link mesenchymal-epithelial transition to radial intercalation in the mouse axial mesoderm; Authors - Marissa L Gredler
mouse	IF	Cell Death Differ; The evolutionary history of the polyQ tract in huntingtin sheds light on its functional pro-neural activities.; Authors - Raffaele Iennaco
human	WB,IF	J Am Soc Nephrol; Crumbs2 Is an Essential Slit Diaphragm Protein of the Renal Filtration Barrier.; Authors - Annika Möller-Kerutt