MPP2 Polyclonal antibody

MPP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13589-1-AP

Synonyms

Discs large homolog 2, DLG2, MAGUK p55 subfamily member 2, MPP2, Protein MPP2

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Validation Data Gallery

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  WB analysis of mouse eye using 13589-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13589-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse eye using 13589-1-AP

  IP result of anti-MPP2 (IP:13589-1-AP, 4ug; Detection:13589-1-AP 1:2000) with mouse eye tissue lysate 2260 ug.

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  IHC staining of mouse brain using 13589-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13589-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse eye tissue, mouse retina tissue
• Positive IP detected in: mouse eye tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13589-1-AP targets MPP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MPP2 fusion protein Ag4223
• Full Name: membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
• Calculated Molecular Weight: 576 aa, 65 kDa
• Observed Molecular Weight: 65 kDa, 60 kDa
• GenBank Accession Number: BC030287
• Gene Symbol: MPP2
• Gene ID (NCBI): 4355
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Membrane protein palmitoylated 2 (MPP2) is a member of the membrane-associated guanylate kinase (MAGUK) family. MPPs can form diverse protein complexes at the cell membranes and play an important role in cell structure, polarity and cell adhesion. MPP2 is a novel postsynaptic scaffold protein that binds to the synaptic cell adhesion molecule SynCAM1. MPP2 also is a component of AMPA receptor-associated protein complexes.