MPI Monoclonal antibody

MPI Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, Mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3B10A6

Cat no : 68417-1-Ig

Synonyms

FLJ39201, Mannose 6 phosphate isomerase, mannose phosphate isomerase, MPI, Phosphohexomutase, Phosphomannose isomerase, PMI, PMI1

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Validation Data Gallery

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  WB analysis using 68417-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68417-1-Ig (MPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of rat brain using 68417-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68417-1-Ig (MPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 68417-1-Ig

  IP result of anti-MPI (IP:68417-1-Ig, 4ug; Detection:68417-1-Ig 1:2000) with Jurkat cells lysate 2280 ug.

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  IF Staining of NIH/3T3 using 68417-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using MPI antibody (68417-1-Ig, Clone: 3B10A6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, rat brain tissue, A549 cells, LnCaP cells, Jurkat cells, K-562 cells, pig brain tissue
• Positive IP detected in: Jurkat cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68417-1-Ig targets MPI in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.

• Tested Reactivity: Human, Rat, Pig, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MPI fusion protein Ag5475
• Full Name: mannose phosphate isomerase
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: BC046357
• Gene Symbol: MPI
• Gene ID (NCBI): 4351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.