MPI Polyclonal antibody

• Featured Product
• KD/KO Validated

MPI Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 14234-1-AP

Synonyms

Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase

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Validation Data Gallery

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  WB analysis using 14234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14234-1-AP (MPI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14234-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14234-1-AP (MPI antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14234-1-AP

  IP result of anti-MPI (IP:14234-1-AP, 4ug; Detection:14234-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IF Staining of NIH/3T3 using 14234-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 14234-1-AP (MPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 14234-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MPI antibody (14234-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

14234-1-AP targets MPI in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MPI fusion protein Ag5475
• Full Name: mannose phosphate isomerase
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: BC046357
• Gene Symbol: MPI
• Gene ID (NCBI): 4351
• RRID: AB_2146302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nature; Mannose impairs tumour growth and enhances chemotherapy.; Authors - Pablo Sierra Gonzalez ; KD Validated
rat	WB	Mil Med Res; D-mannose alleviates intervertebral disc degeneration through glutamine metabolism; Authors - Zheng-Lin Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000