MPG Polyclonal antibody, PBS Only

MPG Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11481-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 11481-2-AP (same clone as 11481-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 11481-2-AP (same clone as 11481-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 11481-2-AP (same clone as 11481-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

  IP result of anti-MPG (IP:11481-2-AP, 4ug; Detection:11481-2-AP 1:300) with HepG2 cells lysate 2200 ug. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11481-2-AP (same clone as 11481-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11481-2-AP (MPG antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

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Product Information

11481-2-PBS targets MPG in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MPG fusion protein Ag2034
• Full Name: N-methylpurine-DNA glycosylase
• Calculated Molecular Weight: 293 aa, 32 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC014991
• Gene Symbol: MPG
• Gene ID (NCBI): 4350
• RRID: AB_2282144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726].