MOV10 Polyclonal antibody

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  WB analysis using 10370-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10370-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10370-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Hepg2 using 10370-1-AP

  IP result of anti-MOV10 (IP:10370-1-AP, 4ug; Detection:10370-1-AP 1:300) with Hepg2 cells lysate 3000 ug.

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  IHC staining of human prostate hyperplasia using 10370-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10370-1-AP (MOV10 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 10370-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10370-1-AP (MOV10 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10370-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MOV10 antibody (10370-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

MOV10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10370-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

KIAA1631, Helicase MOV-10, gb110, fSAP113, EC:3.6.4.13

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, human liver tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: Hepg2 cells
• Positive IHC detected in: human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

10370-1-AP targets MOV10 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOV10 fusion protein Ag0384
• Full Name: Mov10, Moloney leukemia virus 10, homolog (mouse)
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 110-115 kDa
• GenBank Accession Number: BC002548
• Gene Symbol: MOV10
• Gene ID (NCBI): 4343
• RRID: AB_2297897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HCE1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

Publications

Species	Application	Title
human	WB	Neuron; A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation.; Authors - Banerjee Sourav S
		Mol Cell; Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.; Authors - Ameya P Jalihal
human	WB	Mol Cell; MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs.; Authors - Lea H Gregersen ; KD Validated
human	WB,IP	Nat Struct Mol Biol; Capped small RNAs and MOV10 in human hepatitis delta virus replication.; Authors - Haussecker Dirk D ; KD Validated
human	WB,IF	PLoS Genet; MOV10 RNA helicase is a potent inhibitor of retrotransposition in cells.; Authors - Goodier John L JL
human	IF	PLoS Pathog; APOBEC3G and APOBEC3F require an endogenous cofactor to block HIV-1 replication.; Authors - Han Yanxing Y